Thermo Fisher Scientific CD50/ICAM3 Monoclonal Antibody (ICAM3, 1019)
CD50/ICAM3 단클론 항체로 인간 ICAM3 단백질을 특이적으로 인식합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. Protein A/G로 정제된 고순도 항체이며, 액상 형태로 4°C에서 안정적으로 보관 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ICAM3, 1019 |
| Immunogen | Recombinant human ICAM3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes that serves as a counter-receptor for LFA-1 integrin. It functions as an adhesive molecule mediating contact between T cells and antigen-presenting cells, regulates LFA-1 affinity for ICAM-1, and induces T cell activation and proliferation. ICAM-3 plays an essential role in initiating immune responses on both T cells and antigen-presenting cells and interacts with CD209 (DC-SIGN), facilitating communication between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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