Thermo Fisher Scientific PU.1 Monoclonal Antibody (E.388.3)
PU.1 단백질을 인식하는 Thermo Fisher Scientific의 E.388.3 단클론 항체. Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 적합. 인간 및 마우스 반응성, 고순도 친화 크로마토그래피 정제. 연구용으로만 사용 가능.
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Applications 및 권장 희석 배율
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:150–1:200 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.388.3 |
| Immunogen | Synthetic peptide corresponding to the sequence of human PU.1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 8 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10986949 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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