Thermo Fisher Scientific IL-4 Monoclonal Antibody (8D4-8), PE-Cyanine7, eBioscience
인간 IL-4를 인식하는 Mouse IgG1 단클론 항체로, PE-Cyanine7 형광체가 결합된 제품입니다. Flow Cytometry 및 ELISA 분석에 적합하며 0.25 µg/test로 사용 가능합니다. 광산화에 민감하므로 암조건에서 보관하며, 4°C에서 보존합니다.
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Thermo Fisher Scientific IL-4 Monoclonal Antibody (8D4-8), PE-Cyanine7, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
- ELISA (Capture antibody)
Tested Dilution
- ≤ 0.25 µg/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D4-8 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469676 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- APC
- eFluor 450
- PE
- Request custom conjugation
Product Specific Information
Description:
The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15–19 kDa cytokine secreted by Th2 cells.
Applications Reported:
Used as a capture antibody for human IL-4 ELISA and for intracellular staining in flow cytometric analysis.
Applications Tested:
Validated by intracellular flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended usage ≤ 0.25 µg/test (final volume 100 µL). Cell number may range from 10⁵ to 10⁸ cells/test. Optimize titration for best performance.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
488–561 nm excitation; 775 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the IL-4 receptor. It shares overlapping functions with IL-13 via receptor binding. STAT6 mediates IL-4 signaling and immune regulation. The IL-4 gene is part of a cytokine cluster (IL-3, IL-5, IL-13, CSF2) on chromosome 5q, closely located near IL-13 and regulated by long-range elements. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures.
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