Thermo Fisher Scientific CABIN1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues in Human CABIN1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2719547

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

Calcineurin binding protein (Cabin-1) and the corresponding rat homolog, designated Cain, are widely expressed nuclear phosphoproteins that regulate the serine/threonine phosphotase activity of calcineurin and influence T cell signaling and apoptosis. Calcineurin is required for the transcriptional activation of cytokines and the activation of various transcription factors, including NFAT, NFκB and AP-1, involved in T cell receptor (TCR)-mediated signaling. The regulation of calcineurin depends on the changes in intracellular calcium concentrations and the activity of protein kinase C. TCR activation results in PKC inducing the hyperphosphorylation of Cabin-1, which facilitates the high affinity binding of Cabin-1 to calcineurin. This complex formation, in turn, inhibits calcineurin activity and attenuates TCR-mediated signaling. Cabin-1 also associates directly with MEF-2 proteins, a family of transcription factors that regulate apoptosis signaling in T cells. This association between Cabin-1 and MEF-2 leads to the inhibition of MEF-2-mediated gene transcription and the inhibition of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.