Thermo Fisher Scientific ASH2L Monoclonal Antibody (OTI7D11), TrueMAB
인간 ASH2L 단백질을 표적으로 하는 마우스 단클론 항체로, Western blot 및 IHC(P) 실험에 사용 가능. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도, PBS/BSA/글리세롤 버퍼에 보관. 연구용으로만 사용.
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Applications
- Western Blot (WB): 1:2,000
- Immunohistochemistry (Paraffin) (IHC (P)): 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7D11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 347–628 of human ASH2L produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1 (isoform 1), containing a nuclear localization signal and PHD finger motif, suggesting a transcription regulatory role.
Predicted molecular weight: 68.7 kDa
Alternative splicing may result in isoforms at 60 kDa and 56.5 kDa.
Human ASH2L shares:
- 60% homology with Drosophila ash2, which regulates gene expression during development
- 90% homology with mouse ASH2L
Expression profile:
- Predominantly expressed in adult heart and testis, fetal lung and liver
- Low expression in adult lung, liver, kidney, prostate, and peripheral leukocytes
- Detected in leukemia cell lines (e.g., K562, Hel, Dami) with erythroid and megakaryocytic potential
Differentiation inducers such as phorbol ester and hemin alter expression patterns, indicating ASH2L’s role in hematopoiesis and leukemia association.
It may interact with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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