Thermo Fisher Scientific SHH Monoclonal Antibody (OTI3A2), TrueMAB
Human SHH 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot 및 Immunocytochemistry에 사용 가능. 재조합 단백질(aa 24-197)을 면역원으로 제작되었으며, carrier-free 형태로 제공되어 conjugation 실험에 적합. -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A2 |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 24–197 of human SHH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Sonic Hedgehog (SHH) is expressed during embryogenesis and plays a critical role in early developmental patterning, including the ventral neural tube, limb axis, and somite formation.
The SHH precursor undergoes autocatalytic cleavage, producing an N-terminal signaling domain and a C-terminal processing domain. The C-terminal portion attaches a cholesterol moiety to the N-terminal fragment, restricting its diffusion and localization to the cell surface.
Defects in SHH or its signaling pathway are associated with holoprosencephaly (HPE) and may contribute to VACTERL syndrome, characterized by multiple congenital anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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