Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 토끼 다클론 항체로 Western blot, IHC, ICC, Flow, ELISA 등에 사용 가능. 인간 및 생쥐 반응성. Affinity 정제, 액상 형태, -20°C 보관. 세포 간 tight junction 연구용으로 적합.
- 카탈로그번호
- 600-401-GU7x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ZO-1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.05 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human ZO-1. Blast analysis of the sequence of the immunogen shows 100% identity with human.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins associate with claudin, occludin, and F-actin at tight junction strands, providing a linkage between the actin cytoskeleton and the tight junction.
ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, while ZO-1 is also expressed at cell junctions of cardiac myocytes.
ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.