Thermo Fisher Scientific Histone H3, phosphorylated (pSer10) Recombinant Mouse Monoclonal Antibody (rPHH3, 6824)
Histone H3의 Ser10 인산화 형태를 인식하는 Recombinant Mouse Monoclonal Antibody로, IHC(P) 분석에 적합합니다. HEK293 세포에서 발현되었으며, Protein A/G로 정제되었습니다. 액상 형태로 제공되며, -20°C 이하에서 보관 가능합니다.
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Applications
Tested Application
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rPHH3, 6824 |
| Immunogen | A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histone proteins, it forms the nucleosome structure that organizes DNA.
Histones play essential roles in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes various post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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