Thermo Fisher Scientific Histone H3, phosphorylated (pSer10) Recombinant Rabbit Monoclonal Antibody (PHH3, 471R)
Histone H3 (pSer10)을 인식하는 재조합 토끼 단클론 항체로, 인산화된 히스톤 H3 검출에 적합합니다. IHC(P) 실험에 최적화되어 있으며, HEK293 세포에서 발현된 IgG 형식의 항체입니다. 단백질 A/G로 정제되었으며 보존제가 포함되지 않습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PHH3, 471R |
| Immunogen | A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with four core histone proteins, H3 binds to DNA to form the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or facilitate the binding of specific transcription factors.
The N-terminal tail of histone H3 can undergo modifications such as methylation, acetylation, and phosphorylation, influencing various cellular processes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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