Thermo Fisher Scientific IL-6 Monoclonal Antibody (MP520F3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

View 2 publications 2 publications

Neutralization (Neu)

0.005-0.025 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

MP520F3

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609615

Product Specific Information

In Western blots, no cross-reactivity with recombinant human (rh) IL-6, recombinant porcine IL-6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.