Thermo Fisher Scientific Phospho-p38 MAPK alpha (Tyr323) Polyclonal Antibody
Phospho-p38 MAPK alpha (Tyr323)에 특이적인 rabbit polyclonal antibody로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 비인산화 형태에는 반응하지 않습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAPK14 (Accession Q16539), corresponding to amino acid residues around phosphorylated Tyr323. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816480 |
Product Specific Information
This antibody detects endogenous levels of p38 MAPK only when phosphorylated at Tyrosine 323.
Target Information
MAPK14 (p38 alpha) belongs to the stress-activated protein kinase (SAPK) class of MAPKs. The p38 MAPK cascade regulates cellular responses to stress, inflammation, and other stimuli.
p38 MAPK is inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation at Thr-Gly-Tyr motifs.
There are four isoforms of p38 MAPK, differing in tissue expression and affinity for regulators.
Upon exposure to TNF, IL-1, heat shock, or other stimuli, MAPK kinase-3/6 phosphorylates p38 MAPK at Thr180 and Tyr182, leading to activation.
Activated phospho-p38 MAPK subsequently phosphorylates transcription factors such as ATF-2, CHOP-1, and MEF-2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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