Thermo Fisher Scientific MPR 300 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Reconstitute in 100 µL of sterile water.
• Centrifuge to remove any insoluble material.
• The peptide is homologous in rat and mouse.
• Specificity of this antibody: MPRI.

Target Information

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are transported in the cell by approximately 300 kDa cation-independent mannose 6-phosphate receptors (CI-MPR).
The biosynthetic cycle involves MPR transport of newly synthesized lysosomal enzymes from the trans-Golgi network to late endosomes or early lysosomes.
The endocytic cycle moves extracellular lysosomal enzymes from the plasma membrane to early endosomes via clathrin-coated vesicles.
MPRs cycle between these compartments every 3 hours, with steady-state distribution mainly in late endosomes, fewer in the trans-Golgi network, and about 10% at the cell surface.
MPR also binds type II insulin-like growth factor (IGF-II) and can bind both man6P and IGF-II simultaneously.
A soluble ~240 kDa truncated form of MPR, representing the extracellular domain, circulates in serum and retains ligand-binding capability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.