Thermo Fisher Scientific EGFR Monoclonal Antibody (OTI3F2), TrueMAB
EGFR 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot 및 IHC(P)에서 사용 가능. 인간 EGFR(aa 900-1210) 재조합 단백질로부터 유래된 면역원 사용. 동결건조 형태로 제공되며, PBS(pH 7.3, 8% 트레할로스) 용액에 보관. 연구용으로 EGFR 신호전달 및 발현 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 900–1210 of human EGFR produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR has been associated with multiple cancer types, making it a key therapeutic target.
The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB receptor family, which includes ERbB2, ERbB3, and ERbB4.
EGFR activation involves ligand-induced dimerization and autophosphorylation, leading to downstream signaling. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, and esophagus, as well as in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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