Thermo Fisher Scientific CCRL2 Recombinant Rat Monoclonal Antibody (BZ5B8)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Specificity:
This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding.

Preparation:
This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide sequence is NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1–24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Target Information

CCRL2 (C-C chemokine receptor-like 2)
Also known as chemokine receptor X, human chemokine receptor (HCR), CRAM-A, or CRAM-B. It is a seven-pass transmembrane protein expressed by monocytes, neutrophils, and dendritic cells of immune tissues (predominantly spleen, fetal liver, bone marrow, and lymph node).

CCRL2 shares over 40% homology with other C-C chemokine receptors but lacks the conserved DRY motif, which is important for signaling and ligand-binding. C-C chemokine receptors are G-protein coupled, seven-pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.