Thermo Fisher Scientific TPPP Polyclonal Antibody
Rabbit polyclonal antibody against human TPPP. Validated for WB and IHC-P. High specificity and affinity purified. Suitable for detecting TPPP involved in microtubule polymerization. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TPPP. Recombinant protein control fragment (Product #RP-96955). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2648851 |
Product Specific Information
Immunogen sequence:
SLESEGAGEG AAASPELSAL EEAFRRFAVH GDARATGREM HGKNWSKLC
Sequence identity:
- Mouse: 90%
- Rat: 92%
Target Information
Tubulin family members are globular proteins essential for microtubule assembly. Microtubules are structural components involved in mitosis, cytokinesis, and vesicle transport.
TPPP (Tubulin polymerization-promoting protein), also known as p24 or p25, is a 219 amino acid protein localized in the perinuclear cytoplasm. It may form dimers and promotes tubulin polymerization into double-walled tubules or stabilized aggregates. Overexpression of TPPP disrupts mitotic spindle formation and nuclear envelope breakdown.
TPPP is phosphorylated by TPK II and encoded by a gene on human chromosome 5. It plays roles in microtubule polymerization, bundling, and stabilization, maintaining microtubule network integrity, and is involved in mitotic spindle assembly and nuclear envelope breakdown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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