Thermo Fisher Scientific SBP2 Polyclonal Antibody
Human SBP2 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P) 적용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. PBS/glycerol 완충액에 보관하며 장기 저장 시 -20°C 권장. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SBP2. Recombinant protein control fragment (Product #RP-93081). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2646972 |
Product Specific Information
Immunogen sequence:
SEGIKLSADV KPFVPRFAGL NVAWLESSEA CVFPSSAATY YPFVQEPPVT EQKIYTEDMA FGASTFPPQY LSSEITLHPY AYSPYTLDST QNVYSVPGSQ YLYNQPSCYR GFQTVKHRNE NT
Highest antigen sequence identity to the following orthologs:
- Mouse: 69%
- Rat: 69%
Target Information
Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3' untranslated region of the mRNA to decode the UGA codon as Sec. SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex essential for Sec insertion into selenoproteins.
Purified SBP-2 interacts specifically with the SECIS element in phospholipid hydroperoxide glutathione peroxidase mRNA. It shows binding activity in liver, testis, and hepatoma cells. SBP-2 binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1.
A second domain located N-terminal to the RNA binding domain, required for Sec insertion, allows SBP-2 to associate with the ribosomal fraction of cells. SBP-2 preferentially stimulates incorporation directed by selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements. SBP-2 may also have a distinct role in selecting ribosomes for Sec insertion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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