Thermo Fisher Scientific ATG16L1 Polyclonal Antibody
ATG16L1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot 및 ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고순도 항체. 세포 자가포식 연구에 적합.
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Thermo Fisher Scientific ATG16L1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid peptide from near the center of human ATG16 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months; for long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152493 |
Product Specific Information
- Suggested positive control: HeLa cell lysate
- PA5-20489 can be used with blocking peptide PEP-0609
Target Information
Autophagy related protein 16L (ATG16L) is encoded by the ATG16L gene located on chromosome 2 in humans. Also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate and oligomerize with ATG16L to form the 800 kDa Atg16L complex, which facilitates LC3 lipidation to phosphatidylethanolamine (PE), serving as a key marker for autophagy.
The ATG16L complex, along with ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until autophagosome completion.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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