Thermo Fisher Scientific FBXL20 Polyclonal Antibody
FBXL20 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB 및 IHC에 적합. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태. 신경 시냅스 조절 연구에 유용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid peptide near the amino terminus of the human SCRAPPER |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153346 |
Product Specific Information
- A suggested positive control is A-20 cell lysate.
- PA5-20486 can be used with blocking peptide PEP-0606.
Target Information
Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases.
Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at presynaptic membrane.
Scrapper has orthologs in C. elegans, D. melanogaster, and mammals, suggesting that it functions as an important membrane-localized E3 ligase in various species.
Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission.
This identifies protein degradation as a mechanism for holding synaptic communication in check.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-20486_FBXL20_Q96IG2-1_Rabbit.svg, PA5-20486_FBXL20_Q96IG2-1_Rabbit_PDP.jpeg)
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