Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PerCP-eFluor 710, eBioscience
Mouse IL-13을 인식하는 eBio13A 단클론 항체로, PerCP-eFluor 710 형광체가 결합된 제품입니다. Flow cytometry용으로 검증되었으며, Th2 세포 분석에 적합합니다. 488 nm 청색 레이저로 여기되며, 710 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), PerCP-eFluor 710
Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular staining)
- Tested Dilution: ≤ 0.25 µg/test
A test is defined as the amount of antibody that stains cells in 100 µL volume (10⁵–10⁸ cells/test).
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor 710, eBioscience |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio13A |
| Conjugate | PerCP-eFluor 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218893 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 660
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- Request custom conjugation
Product Specific Information
The eBio13A antibody reacts with mouse IL-13, a cytokine mainly produced by Th2 cells and antigen-primed CD8 T cells. IL-13 plays key roles in allergic inflammation, parasite clearance, and modulation of macrophage activity by reducing pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12).
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Th2-polarized mouse splenocytes
- Recommended Use: ≤ 0.25 µg/test, titrate for optimal performance
Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Excitation: 488 nm (Blue Laser)
- Emission: 710 nm
- Recommended Filters: 685 LP dichroic mirror + 710/40 band pass filter (695/40 acceptable alternative)
Stability:
Conjugated antibodies are stable when samples are fixed with freshly prepared 2% formaldehyde overnight at 4°C.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It down-regulates macrophage activity and suppresses pro-inflammatory cytokine production. IL-13 is involved in allergen-induced asthma independent of IgE and eosinophils, and regulates B cell maturation and IgE isotype switching.
The IL13 gene is located on chromosome 5q within a cytokine gene cluster near IL4, IL3, IL5, and CSF2.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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