Thermo Fisher Scientific Cystatin B Monoclonal Antibody (CPTC-CSTB-3)
Cystatin B 단백질을 인식하는 mouse monoclonal antibody로, Western blot 및 IHC(P)에서 검증됨. 단백질 A/G로 정제된 비결합형 액상 항체이며, 4°C에서 보관. 연구용으로 인간 시료에 반응.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CPTC-CSTB-3 |
| Immunogen | Recombinant full-length human Cystatin B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Cystatin A (also designated STF1, STFA, stefin A or cystatin AS) and Cystatin B (also designated PME, CST6, STFB, CPI-B, stefin B, and liver thiol proteinase inhibitor) are thiol protease inhibitors that form complexes with Papain and the cathepsins B, H, and L.
Cystatin A, a cytoplasmic protein, is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance.
Cystatin B protects against intracellular proteases leaking out of lysosomes and is primarily expressed in heart, liver, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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