Thermo Fisher Scientific proBNP Polyclonal Antibody, Biotin
Biotin으로 표지된 proBNP 폴리클로날 항체로 다양한 면역분석(WB, IHC, ELISA 등)에 사용 가능. 사람, 마우스, 설치류, 영장류 반응성. 친화 크로마토그래피로 정제된 고순도 항체로 심부전 및 심장 기능 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–150 on human proBNP protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
In cardiac tissue, brain natriuretic peptide (BNP) is synthesized as a 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa signal peptide and a 108 aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76 aa amino-terminal NT-proBNP and biologically active 32 aa BNP hormone molecule. Both proBNP and NT-proBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers for cardiac complications in patients with heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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