Thermo Fisher Scientific IFN gamma Polyclonal Antibody, Biotin

Applications

• ELISA (ELISA): Assay-dependent

Product Specifications

Product Specific Information

• Reconstitute with 1 mL of deionized water for a final concentration of 0.25 mg/mL.
• PBOIFNGBI targets IFN gamma in ELISA applications and shows reactivity with bovine samples.
• The immunogen is recombinant bovine IFN gamma.
• Detects IFN gamma with a predicted molecular weight of approximately 17 kDa.
• Successfully paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody PBOIFNGI.
• Typical dilutions for sandwich ELISA: 1 µg/mL for coating and 0.125–0.25 µg/mL for detection.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein consisting of two 146 amino acid subunits, functionally existing as a homodimer (~45 kDa). On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
The biological activity is highly species-specific; human IFN gamma does not cross-react with mouse IFN gamma.
Functions include:

• Antiviral and antitumor activity
• Induction of class I and II MHC
• Macrophage activation
• Enhanced immunoglobulin secretion by B lymphocytes

IFN gamma acts via binding to IFN gamma receptor I and II, activating the JAK-STAT pathway. It shows ~40% sequence homology between human and mouse IFN gamma.
Expression is upregulated by IL2, FGF basic, and EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.