Thermo Fisher Scientific IFN gamma Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Bovine IFN gamma

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.25 mg/mL

Purification

purified

Storage buffer

PBS

Contains

proprietary preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979934

Product Specific Information

Reconstitute with 1mL of deionized water for a final concentration of 0.25mg/mL.

PBOIFNGBI targets IFN gamma in ELISA applications and shows reactivity with Bovine samples.

The PBOIFNGBI immunogen is recombinant Bovine IFN gamma.

PBOIFNGBI detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.

The PBOIFNGBI polyclonal biotinylated bovine IFNg antibody has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody PBOIFNGI. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.