Thermo Fisher Scientific proBNP Polyclonal Antibody, FITC
FITC로 표지된 proBNP 폴리클로날 항체로, 인간 및 설치류 시료에서 proBNP 단백질 검출에 사용됩니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합하며, 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 100–150 on human proBNP protein |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
In cardiac tissue, brain natriuretic peptide (BNP) is synthesized as a 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 amino acid signal peptide and a 108 amino acid pro-BNP. Proteolytic digestion of pro-BNP results in formation of a 76 amino acid amino-terminal NT-proBNP and a biologically active 32 amino acid BNP hormone molecule. Both proBNP and NT-proBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications in patients with heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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