Thermo Fisher Scientific c-Raf Polyclonal Antibody
Human c-Raf 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P) 및 ICC/IF에 적합. 항원 친화 크로마토그래피로 정제되었으며 PBS buffer에 40% glycerol 포함. 연구용으로 세포 신호전달 및 MAPK 경로 분석에 활용 가능.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:20–1:50
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human RAF1. Recombinant protein control fragment (Product #RP-102057) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.03 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789187 |
Product Specific Information
Immunogen sequence:
FRCQTCGYKF HEHCSTKVPT MCVDWSNIRQ LLLFPNSTIG DSGVPALPSL TMRRMRESVS RMPVSSQHRY STPHAFTFNT SSPSSEGSLS QRQRSTSTPN VHMVSTTLPV DSRMIEDAIR SHSESASPSA LSSSPNNLSP TGWSQ
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of Ras family GTPases. Once activated, Raf-1 phosphorylates MEK1 and MEK2, which activate ERK1 and ERK2. Activated ERKs regulate cell division, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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