
Thermo Fisher Scientific CD166 Polyclonal Antibody
CD166(ALCAM) 단백질을 인식하는 Thermo Fisher Scientific의 고트 폴리클로날 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 연구용으로 세포 부착 및 이동 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.2 µg/mL | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg per million cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166 (Trp28–Lys527) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607383 |
Product Specific Information
In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM-1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, a member of the immunoglobulin receptor subfamily with five immunoglobulin-like domains (VVC2C2C2) in the extracellular region.
It binds to T-cell differentiation antigen CD6 and is involved in cell adhesion and migration processes.
Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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