Thermo Fisher Scientific Actin Muscle Monoclonal Antibody (HHF35)
근육 특이적 알파 및 감마 액틴을 인식하는 마우스 모노클로날 항체로 다양한 종에서 사용 가능. WB 및 IHC 등 여러 응용에 적합하며, 근육 및 근육 유래 세포의 표지자로 널리 활용. 단백질 A로 정제된 액체형 항체로 4°C에서 보관.
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Thermo Fisher Scientific Actin Muscle Monoclonal Antibody (HHF35)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 9 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Cat, Human, Mouse, Non-human primate, Rabbit, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HHF35 |
| Immunogen | SDS extracted protein fraction of human myocardium |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154239 |
Product Specific Information
This antibody recognizes muscle-specific alpha and gamma actin (42 kDa) in various species.
It stains skeletal, smooth, and myocardial cells as well as myoepithelial cells and pericytes of small vessels.
It is a widely used marker of muscle and muscle-derived cells.
- Western Blot: Reducing conditions recommended
- Immunohistochemistry (Paraffin): Antigen retrieval steps generally not required; pepsin or trypsin pretreatment recommended for arterial smooth muscle cells or myoepithelial cells
Target Information
Actin belongs to a highly conserved family of proteins involved in cell motility, structure, and integrity.
Alpha, beta, and gamma actin isoforms have distinct roles:
- Alpha actins: Major constituent of the contractile apparatus
- Beta and gamma actins: Regulate cell motility
This actin is an alpha actin found in skeletal muscle.
Mutations in this gene are associated with various congenital myopathies, including nemaline myopathy type 3 and congenital myopathy with fiber-type disproportion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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