Thermo Fisher Scientific Galectin 3 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific Galectin 3 Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Galectin-3. Anti-Human Galectin-3-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hGalectin-3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Galectin-3 (500-P246) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hGalectin-3.

Western Blot:
To detect hGalectin-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hGalectin-3 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P246BT-1MG will be provided as 2 × 500 µg.

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus, acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes/macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: 500-P246BT-500UG_Galectin_3_P17931-1_Rabbit.svg 및 500-P246BT-500UG_Galectin_3_P17931-1_Rabbit_PDP.jpeg)