Thermo Fisher Scientific Phospho-SLP76 (Tyr128) Polyclonal Antibody
Phospho-SLP76 (Tyr128) 폴리클로날 항체로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ELISA에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 기반 버퍼에 보관되며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| ELISA | 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human SLP-76 around the phosphorylation site of Y128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607972 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat.
The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice.
The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains:
- NH2-terminus: Contains an acidic region including a PEST domain and several tyrosine residues phosphorylated following TCR ligation.
- Central region: Proline-rich domain.
- COOH-terminus: SH2 domain.
SLP-76 interacts with multiple proteins constitutively and inducibly following receptor ligation, functioning as an adaptor or scaffold protein.
Studies using SLP-76 deficient T cell lines or mice have shown that SLP-76 plays a positive role in T cell development and activation, as well as mast cell and platelet function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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