Thermo Fisher Scientific CD257 (BAFF, BLyS) Monoclonal Antibody (1D6), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats:

• APC
• FITC
• PE
• Request custom conjugation

Product Specific Information

Description:
The 1D6 monoclonal antibody reacts with human BLyS (B lymphocyte stimulator), also known as BAFF (B cell activating factor from the TNF family), TALL-1, THANK, and zTNF4. BLyS is a type II membrane protein that exists in both membrane-bound and soluble forms. Expression of membrane-bound BLyS on monocytes is regulated by IFN-γ.
BLyS functions as a potent B cell growth factor and costimulator of immunoglobulin production. BLyS transgenic mice develop severe B cell hyperplasia and autoimmune lupus-like disease. Data from animal models suggest a role for BLyS in human autoimmune diseases.
Receptors identified for BLyS include BCMA, TACI, and BR3. BLyS and APRIL can form active heterotrimers present in serum samples from patients with systemic immune-based rheumatic diseases.

Applications Reported:
Flow cytometric analysis, Western blotting, and immunohistochemical staining.

Applications Tested:
Tested by flow cytometric analysis of human BLyS transfected cells. Recommended ≤1 µg/test (100 µL final volume). Optimal titration is advised for specific assays.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

BAFF is a membrane-bound cytokine expressed by B cells. The soluble form binds to receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. It is a potent B cell activator, promoting proliferation and differentiation. BAFF is also expressed by T lymphocytes and supports T cell activation and survival. Multiple isoforms exist due to alternative splicing and glycosylation.

For Research Use Only. Not for use in diagnostic procedures.