Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (7-239), PE, eBioscience

Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (7-239), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 2 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

This 7-239 monoclonal antibody reacts with human CD169 (Siglec-1, Sialoadhesin), a type I transmembrane protein belonging to the sialic acid binding Ig-like lectin (Siglec) family.
CD169 is expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen. It binds glycoproteins and glycolipids containing N-acetylneuraminyl α2-3-galactose.
Expression can be induced on CD14+ monocytes by interferon-α and interferon-γ, mediating HIV-1 infection. CD169 also participates in antigen presentation to invariant NK T cells.

This antibody inhibits erythrocyte binding to CD169-expressing cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume). Cell number may range from 10^5 to 10^8 cells/test.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The Siglec family consists of membrane-bound lectins (immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions.
Family members include sialoadhesin, CD22, and CD33.
Sialoadhesin is a macrophage-specific adhesion molecule expressed in chronic inflammation and tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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