Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE, eBioscience
인간 CD163 단백질을 표적하는 단클론 항체로, PE 형광표지되어 흐름세포분석에 적합합니다. 조직 대식세포 및 단핵세포에서 CD163 발현 분석에 사용되며, 높은 특이성과 신뢰성 있는 결과를 제공합니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 12-1639-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE, eBioscience
Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE, eBioscience™
Applications
- Immunohistochemistry (Frozen): Not tested
- Flow Cytometry (Flow): 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioGHI/61 (GHI/61) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1963571 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Biotin
- Brilliant Violet 421
- Brilliant Violet 711
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family.
CD163 is expressed by tissue macrophages, circulating blood monocytes, and possibly by hematopoietic progenitor cells. It functions as a high-affinity receptor for the hemoglobin-haptoglobin complex to clear hemoglobin from blood. Ligand binding triggers signal transduction leading to IL-6 and IL-10 production.
CD163 expression is induced by IL-6 and IL-10, while proinflammatory stimuli like LPS or TNF-α cause shedding of soluble CD163.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and receptor for the hemoglobin-haptoglobin complex. It protects tissues from oxidative damage and plays a role in iron recycling via endocytosis.
CD163 is expressed exclusively on human monocytes and macrophages, predominantly in late inflammation. It is present on all CD14⁺ monocytes and most CD64⁺ monocytes, showing higher expression on CD16⁺ subsets.
Expression is upregulated by IL-10, IL-6, dexamethasone, and shed by LPS or PMA stimulation. CD163 binding is calcium- and pH-dependent, showing higher affinity for multimeric haptoglobin (HP1F phenotype).
Intracellular signaling involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL-6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures.
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