Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE, eBioscience

Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE, eBioscience™

Applications

• Immunohistochemistry (Frozen): Not tested
• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 711
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Custom conjugation available

Product Specific Information

The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family.
CD163 is expressed by tissue macrophages, circulating blood monocytes, and possibly by hematopoietic progenitor cells. It functions as a high-affinity receptor for the hemoglobin-haptoglobin complex to clear hemoglobin from blood. Ligand binding triggers signal transduction leading to IL-6 and IL-10 production.
CD163 expression is induced by IL-6 and IL-10, while proinflammatory stimuli like LPS or TNF-α cause shedding of soluble CD163.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein and receptor for the hemoglobin-haptoglobin complex. It protects tissues from oxidative damage and plays a role in iron recycling via endocytosis.
CD163 is expressed exclusively on human monocytes and macrophages, predominantly in late inflammation. It is present on all CD14⁺ monocytes and most CD64⁺ monocytes, showing higher expression on CD16⁺ subsets.
Expression is upregulated by IL-10, IL-6, dexamethasone, and shed by LPS or PMA stimulation. CD163 binding is calcium- and pH-dependent, showing higher affinity for multimeric haptoglobin (HP1F phenotype).
Intracellular signaling involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL-6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures.

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