Thermo Fisher Scientific CD73 Monoclonal Antibody (4G4)
인간 CD73 단백질을 인식하는 4G4 클론의 마우스 모노클로날 항체로, WB, IHC, ICC, Flow, IP, Functional Assay 등에 사용 가능. CD73 발현 감소 및 효소 활성 억제 효과. Protein G 정제, PBS+BSA 용액, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
| Immunoprecipitation (IP) | 1:50 |
| Functional Assay (Functional) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G4 |
| Immunogen | Inflamed synovial stroma from rheumatoid arthritis patients |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_3091074 |
Product Specific Information
The monoclonal antibody 4G4 recognizes both membrane-bound and soluble human CD73 (ecto-5-nucleotidase). It reduces CD73 expression on lymphocytes, decreases enzyme activity, and inhibits lymphocyte binding to endothelial cells.
Target Information
CD73 (Ecto-5′-nucleotidase, 5′-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5′-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate.
It is a dimer of two identical 70 kDa subunits attached to the plasma membrane via a glycosyl phosphatidylinositol linkage and serves as a marker of lymphocyte differentiation.
Deficiency of CD73 is associated with various immunodeficiency diseases. The CD73 gene is localized to chromosome 6q14–q21. Defects in this gene can cause calcification of joints and arteries and intestinal tuberculosis. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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