Thermo Fisher Scientific Phospho-Aquaporin 2 (Ser264) Polyclonal Antibody
Aquaporin 2의 Ser264 인산화 형태를 인식하는 Rabbit Polyclonal Antibody. Western blot과 IHC에 적합하며, Human, Mouse, Rat에 반응. Affinity chromatography로 정제된 액상 항체로, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | 1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser264 of rat AQP2 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/mL BSA |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2552697 |
Product Specific Information
This antibody is predicted to react with canine, bovine, chicken, human, mouse, and non-human primate based on 100% sequence homology.
PA5-35387 is specific for the ~29 kDa AQP2 protein phosphorylated at Ser264 and also recognizes the glycosylated form of AQP2 at ~37 kDa.
This antibody contains enough material to conduct approximately 10 mini-Western blots.
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct.
Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans.
A vasopressin-induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells.
Serine-261 has also been identified as a novel phosphorylation site on AQP2, with phosphorylated S261 levels decreasing upon vasopressin treatment, suggesting its involvement in vasopressin-dependent AQP2 trafficking.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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