Thermo Fisher Scientific Phospho-Aquaporin 2 (Ser269) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:100

-

Immunocytochemistry (ICC/IF)

1:200-1:500

-

Immunoprecipitation (IP)

3 µL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 of rat AQP2 conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Aquaporin 2,uniProtId:P34080-1,ncbiNodeId:10114,antigenRange:269,antigenLength:271,antigenImageFileName:PA5-35388_Aquaporin_2_P34080-1_Rabbit.svg,antigenImageFileNamePDP:PA5-35388_Aquaporin_2_P34080-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.25 mg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/mL BSA

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2552698

Product Specific Information

This antibody is predicted to react with bovine, canine and mouse based on 100% sequence homology.

PA5-35388 is specific for the ~29kDa AQP2 protein phosphorylated at Ser269, and also recognizes the glycosylated form of AQP2 at ~37kDa. This antibody contains enough material to conduct 10 mini-Western blots.

Target Information

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.