Thermo Fisher Scientific PPP2R4 Monoclonal Antibody (4D9)
PPP2R4 단백질을 검출하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합함. 인간 시료 반응성, 고순도 Protein G 정제, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4D9 |
| Immunogen | Purified recombinant fragment of human PPP2R4 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538627 |
Product Specific Information
MA5-17156 targets PPP2R4 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human PPP2R4 expressed in E. coli.
MA5-17156 detects PPP2R4, which has a predicted molecular weight of approximately 41 kDa.
Target Information
Protein phosphatase 2A (PP2A) is one of the major Ser/Thr phosphatases involved in negative regulation of cell growth and division. PP2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B.
Regulatory subunits are encoded by diverse genes grouped into the B/PR55, B'/PR61, and B''/PR72 families, conferring distinct enzymatic specificities and intracellular localizations.
The PPP2R4 gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of PP2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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