Thermo Fisher Scientific PSAP Monoclonal Antibody (4D5F4)
Human PSAP 단백질을 인식하는 Mouse monoclonal antibody(4D5F4)로, WB, IHC, ICC, Flow cytometry, ELISA에 적합합니다. Protein G 정제, PBS buffer에 0.05% sodium azide 포함, 1 mg/mL 농도. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4D5F4 |
| Immunogen | Purified recombinant fragment of human PSAP (amino acids: 325–524) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538630 |
Product Specific Information
MA5-17159 targets PSAP in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with Human samples.
The immunogen is a purified recombinant fragment of human PSAP (amino acids 325–524) expressed in E. coli.
MA5-17159 detects PSAP, which has a predicted molecular weight of approximately 58.1 kDa.
Target Information
PSAP is a highly conserved glycoprotein that serves as a precursor for four cleavage products: saposins A, B, C, and D.
Each domain of the precursor protein is approximately 80 amino acids long, with nearly identical cysteine residue placement and glycosylation sites.
Saposins A–D localize primarily to the lysosomal compartment, facilitating the catabolism of glycosphingolipids with short oligosaccharide chains.
The precursor protein exists both as a secretory and an integral membrane protein and exhibits neurotrophic activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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