Thermo Fisher Scientific RAP1A Monoclonal Antibody (5F8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5F8

Immunogen

Purified recombinant fragment of human RAP1A expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538635

Product Specific Information

MA5-17164 targets RAP1A in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17164 immunogen is purified recombinant fragment of human RAP1A expressed in E. Coli.

MA5-17164 detects RAP1A which has a predicted molecular weight of approximately 21kDa.

Target Information

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.