Thermo Fisher Scientific D4-GDI Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/ml

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full-length human D4-GDI protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549847

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta or tonsil tissue.

Target Information

Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.