Thermo Fisher Scientific DOG-1 Polyclonal Antibody
Thermo Fisher Scientific의 DOG-1 Polyclonal Antibody는 인간 DOG1 단백질에 대한 고특이성 항체로, IHC 및 ICC/IF 실험에 사용됩니다. Rabbit IgG 기반의 액상 형태로 제공되며, 위장관 기질종양(GIST) 연구에 적합합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide of human DOG1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549853 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is gastrointestinal stromal tumor.
Target Information
DOG-1, the novel marker, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFRα mutation status.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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