Thermo Fisher Scientific Complement C4b Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Flow Cytometry (Flow)

1 µg/1 million cells

-

ELISA (ELISA)

1:1,000-1:5,000

-

Product Specifications

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Purified human complement C4b.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073831

Product Specific Information

PA1-70042 detects C4b in human samples.

PA1-70042 has been successfully used in flow cytometry, Western blot and ELISA procedures.

The PA1-70042 immunogen is purified human complement C4b.

Target Information

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.