Thermo Fisher Scientific alpha Internexin Recombinant Rabbit Monoclonal Antibody (JU25-03)
Recombinant rabbit monoclonal antibody targeting alpha Internexin. Validated for WB, IHC, ICC/IF, and Flow Cytometry. Offers high specificity, lot-to-lot consistency, and animal-origin-free formulation. Suitable for neuronal development and neurofilame...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU25-03 |
| Immunogen | Synthetic peptide within Human alpha Internexin (aa 394–443) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810085 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and clones are screened to select optimal candidates.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
alpha-Internexin is a Class IV intermediate filament subunit expressed early in neuronal development. It is related to but distinct from neurofilament triplet proteins (NF-L, NF-M, NF-H), sharing similar motifs and intron organization.
Many mature neurons express alpha-Internexin alongside NF-L, NF-M, and NF-H, while some express only alpha-Internexin.
Antibodies to alpha-Internexin are valuable for:
- Studying neuronal types and developmental processes
- Identifying neuronal progenitor cells
- Potential use in brain tumor research
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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