Thermo Fisher Scientific 6x-His Tag Chimeric Recombinant Rat Monoclonal Antibody (3D5)

Applications

• Western Blot (WB): Assay-dependent
• Flow Cytometry (Flow): Assay-dependent
• ELISA (ELISA): Assay-dependent

Product Specifications

Product Specific Information

This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at the N- or C-terminal regions of recombinant proteins to enable purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins using antibodies against the tag sequence, facilitating purification and identification even when specific antibodies for the target protein are unavailable.
His-tagged proteins can be purified efficiently due to the strong and selective chelation between histidine residues and metal ions (e.g., Ni²⁺) on affinity resins such as Ni²⁺-NTA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.