Thermo Fisher Scientific 6x-His Tag Chimeric Recombinant Rat Monoclonal Antibody (3D5)
6x-His 태그 단백질 검출용 랫드 단클론 항체로, C-말단 및 내부 His-tag에 높은 특이성을 가집니다. Western blot, Flow cytometry, ELISA 등 다양한 분석에 사용 가능하며, HEK293 세포에서 발현된 재조합 항체입니다.
- 카탈로그번호
- MA551581
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- Flow Cytometry (Flow): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rat / IgG2c, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3D5 |
| Immunogen | Purified His tagged Fab fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
Product Specific Information
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins.
It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly fused at either the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection or purification of recombinant proteins by fusing a known epitope sequence.
His-tagged proteins can be purified through interaction of histidine residues with metal ions such as Ni²⁺ on Ni-NTA resins, providing strong and selective binding.
His tag-specific antibodies, such as this one, are widely used for detection and co-immunoprecipitation of His-tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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