Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Super Bright 702, eBioscience
Mouse CD62L(L-Selectin)을 인식하는 MEL-14 단클론 항체로, Super Bright 702 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 702 nm에서 방출됩니다. 비특이적 결합 최소화를 위해 Super Bright Staining Buffer 사용을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.03 µg/test | View 28 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEL-14 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717151 |
Available Conjugate Formats
- Alexa Fluor 561
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 555 / 585 / 610-30S / 660-40S
- NovaFluor Red 710
- NovaFluor Yellow 590 / 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 780
- Request custom conjugation
Product Specific Information
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. CD62L mediates lymphocyte rolling on activated endothelium and homing to peripheral lymphoid tissues via interactions with CD34, glycam-1, and MAdCam-1.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤0.03 µg/test (100 µL final volume). Cell number should be optimized (10⁵–10⁸ cells/test).
Super Bright 702 Characteristics:
- Excitation: 405 nm
- Emission: 702 nm
- Recommended filter: 710/50 bandpass
- Sensitive to photo-induced oxidation; protect from light.
- Use Super Bright Complete Staining Buffer (SB-4401) to minimize non-specific polymer interactions.
- Fixation: Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor composed of:
- C-type lectin binding domain
- EGF-like domain
- Two short consensus repeat (SCR) sequences
- Transmembrane and cytoplasmic regions
It is constitutively expressed on most leukocytes, including lymphocytes (except some memory T cells), monocytes, and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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