Thermo Fisher Scientific CD304 (Neuropilin-1) Monoclonal Antibody (3DS304M), APC, eBioscience

Thermo Fisher Scientific CD304 (Neuropilin-1) Monoclonal Antibody (3DS304M), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 2 publications

Product Specifications

Additional Formats

• eFluor 450
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The monoclonal antibody 3DS304M recognizes mouse CD304 (Neuropilin-1), a type 1 transmembrane glycoprotein expressed on multiple cell types including regulatory T cells (Treg), NKT cells, dendritic cells, stem cells, neurons, endothelial cells, and neoplastic cells.
Neuropilin-1 acts as a co-receptor for type 3 semaphorins in growing neurons and contributes to angiogenesis as part of a VEGF receptor complex in endothelial cells. In mice, Neuropilin-1 is expressed on thymus-derived natural Treg but not on peripherally induced Treg.
Transient expression on recently activated non-regulatory T cells has been observed. Neuropilin-1 serves as a marker for recent thymic emigrants in mouse iNKT cells. It forms a complex with TGF-beta receptors, activating latent TGF-beta and enhancing Smad2/3 signaling.

This antibody recognizes formaldehyde-fixed and methanol-treated epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤ 0.25 µg/test (100 µL final volume). Recommended careful titration for optimal assay performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Neuropilin-1 is a 923 amino acid membrane-bound coreceptor belonging to the neuropilin family. It contains two CUB domains, two F5/8 type C domains, and a MAM domain. Neuropilin-1 forms a heterodimer with Neuropilin-2 (NRP2) and binds to plexin (PLXNB1) to form semaphorin receptors.
It is primarily expressed in the nervous system and plays roles in cardiovascular development, angiogenesis, axon guidance, neuronal circuit formation, cell survival, and migration. Neuropilin-1 also functions as a receptor for semaphorins and VEGF, regulating VEGF-induced angiogenesis.
It may be implicated in advanced prostate carcinoma and breast cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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