Thermo Fisher Scientific PIR-A/B Monoclonal Antibody (10-1-PIR), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

10-1-PIR

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1944406

Product Specific Information

Description: This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating (PIR)-A and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs (ITAMs) which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs (ITIMs); therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection.

Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1 (Product # 46-5978).

Applications Reported: This 10-1-PIR antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 10-1-PIR antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Paired Ig-like receptors of activating (PIR)-A and -B are expressed on B lymphocytes, macrophages, dendritic cells, platelets, granulocytes, and mast cells, but not on T cells, NK cells, and erythrocytes. These cell surface glycoproteins contain six extracellular Ig-like domains with distinct cytoplasmic and transmembrane regions. The PIR-A receptor activates through signaling molecules containing immunoreceptor tyrosine-based activation motifs. In contrast, the PIR-B receptor inhibits through proteins possessing immunoreceptor tyrosine-based inhibitory motifs. PIRs bind MHC class I to modulate immune system homeostasis and cell signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.