Thermo Fisher Scientific Synaptophysin Recombinant Rabbit Monoclonal Antibody (SYP, 4503R)
Synaptophysin 단백질을 인식하는 재조합 토끼 단일클론 항체로, 신경세포 및 신경내분비 종양 표지에 적합. IHC(P)에서 1–2 µg/mL 권장. HEK293 세포에서 발현된 IgG 형식, Protein A/G 정제, 보존제 무첨가 PBS 용액 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SYP, 4503R |
| Immunogen | Recombinant fragment (around aa 224–313) of human Synaptophysin (SYP) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Synaptophysin is a calcium-binding integral membrane glycoprotein localized in presynaptic vesicles of nearly all neurons. It contains four transmembrane domains and forms a complex with dynamin at high calcium concentrations, suggesting a role in synaptic vesicle endocytosis. It also participates in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is widely used as a marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in X-linked mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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