Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (AFKJS-9), APC, eBioscience
ROR gamma(t) 단백질을 인식하는 AFKJS-9 단클론 항체로, 인간, 마우스, 원숭이에 반응합니다. APC로 표지되어 유세포분석(Flow Cytometry)에 최적화되어 있으며, 651/660 nm 파장에서 발광합니다. 세포 내 염색 및 Th17 세포 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 46 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rhesus monkey |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AFKJS-9 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1633425 |
Additional Formats
Product Specific Information
The AFKJS-9 monoclonal antibody reacts with mouse, human, and rhesus monkey ROR gamma (t) protein.
ROR gamma belongs to the retinoic acid-related orphan receptor (ROR) family, including ROR alpha and ROR beta. These ligand-dependent transcription factors are involved in multiple physiological processes.
ROR gamma is expressed in liver, lung, muscle, heart, and kidney. The ROR gamma (t) isoform, expressed in lymphoid cells such as CD4+CD8+ thymocytes, Th17 cells, and LTi cells, differs by three amino acids at the N-terminus. Thus, AFKJS-9 antibody recognizes both ROR gamma and ROR gamma (t).
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometry using mouse thymocytes and the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Refer to BestProtocols® for detailed staining protocols.
- Recommended use: ≤1 µg/test
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
ROR gamma (NR1 Thyroid Hormone-Like receptor) regulates thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis.
It promotes thymocyte survival via Bcl-x(L) expression and is essential for lymph node and Peyer’s patch development.
ROR gamma(t), a thymus-specific isoform, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.
ROR gamma binds as a monomer to response elements containing a GGTCA core motif with an AT-rich sequence.
Expression is found in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle.
RORC isoform ROR gammat (RORgt) is involved in immune regulation and Th17 cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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