Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), FITC, eBioscience
인간 CD19 항원을 인식하는 HIB19 클론 단일클론 항체로, FITC로 결합되어 Flow Cytometry에 최적화됨. B세포 발달 단계 전반에서 발현되는 CD19 검출에 사용. 488 nm 여기, 520 nm 방출 특성. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | View 67 publications |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIB19 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10668005 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, Yellow series (multiple wavelengths)
- eFluor 450, 506
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- NovaFluor Blue, Red, Violet, Yellow series
- Functional Grade
- Custom conjugation available upon request
Product Specific Information
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except terminal plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, Leu13, and MHC class II, CD19 forms a multimolecular complex associated with BCR.
Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B-cell proliferation. The SJ25C1 and HIB19 antibodies recognize overlapping epitopes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells. Use at 5 µL (1 µg) per test (100 µL final volume).
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR), regulating B cell activation and proliferation.
Mutations lead to hypogammaglobulinemia, while overexpression causes B cell hyperactivity. CD19 is a key marker for B cell lineage and an important target in immunological research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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