Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV (395–805)
• Brilliant Violet (421–786)
• eFluor (450, 506, 660)
• Functional Grade
• NovaFluor (Blue, Red, UV, Violet, Yellow series)
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright (436, 600, 645, 702, 780)
• Custom conjugation available upon request

Product Specific Information

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except in terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, and MHC class II, CD19 forms a multimolecular complex associated with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes
• Recommended Usage: ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and may also appear on certain myeloid leukemia cells. CD19 functions as a signal-amplifying coreceptor for the B cell receptor (BCR), lowering the activation threshold for antigen-dependent stimulation. It plays a central role in regulating normal and malignant B cell proliferation. Mutations in CD19 cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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